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100bp
Catalog #: 65-0241
$99.00    

CERTIFICATE OF ANALYSIS

GeneRuler

100bp DNA Ladder 

#65-0241 0.05mg Ready-Load 

Lot: 3500  

Concentration: 0.05mg DNA/ml

Supplied with:  1ml 6X Loading Dye Solution 

Store at -20 ℃ 

Description

GeneRuler 100bp DNA Ladder is prepared from a specially designed plasmid pME-80J3 DNA, containing pUC, λ phage and yeast genome sequences. pME-80J3 DNA was completely digested with Eco1471 and Pvul. The DNA Ladder is dissolved in storage buffer. The DNA Ladder contains the following 11 discrete fragments (in base pairs): 1031, 900, 800, 700, 600, 500, 400, 300, 200, 100, 80. 

Storage Buffer

10mM Tris-HCI(pH7.6), 1mM EDTA. 

6X Loading Dye Solution

0.09% bromophenol blue, 0.09% xylene cyanol FF, 60% glycerol and 60mM EDTA. 

Quality Control Assay Data

Analysis of 0.5ug (1ul) of the DNA Ladder on agarose gel and 1 ug (2ul)- on polyacrylamide gel by ethidium bromide staining generates 11 discrete bands pattern. 

Quality authorized by:        Jurgita Zilinskiene 

RECOMMENDATIONS FOR USE

Loading on agarose gel:

-Prepare the DNA Ladder before loading:

1ul (0.5ug) of the DNA Ladder,

1ul of 6x Loading Dye Solution,

4ul of deionized water;

-vortex gently just prior to use;

-do not heat before loading;

-apply the prepared amount (6ul) of the DNA Ladder on a 5mm lane of agarose gel;

-following electrophoretic separation on gel, visualize the DNA bands by ethidium bromide staining. 

Note

  • One vial 0.05mg) is sufficient for ~ 100 applications.
  • Use 0.1ug (0.2ul) of the DNA Ladder (before dilution) per 1mm of an agarose gel lane width.

 

This product was added to our catalog on Monday 07 December, 2009.
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